RNA Study Using DNA Nanotechnology

Prog Mol Biol Transl Sci. 2016;139:121-63. doi: 10.1016/bs.pmbts.2015.11.004. Epub 2016 Feb 1.

Abstract

Transcription is one of the fundamental steps of gene expression, where RNA polymerases (RNAPs) bind to their template genes and make RNAs. In addition to RNAP and the template gene, many molecules such as transcription factors are involved. The interaction and the effect of these factors depend on the geometry. Molecular layout of these factors, RNAP and gene is thus important. DNA nanotechnology is a promising technology that allows controlling of the molecular layout in the range of nanometer to micrometer scale with nanometer resolution; thus, it is expected to expand the RNA study beyond the current limit.

Keywords: DNA nanostructure; DNA origami; RNA polymerase; atomic force microscopy (AFM); single-molecule imaging.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Base Sequence
  • DNA / chemistry
  • DNA / metabolism*
  • Humans
  • Microscopy, Atomic Force
  • Nanostructures / chemistry
  • Nanotechnology / methods*
  • RNA / chemistry
  • RNA / metabolism*

Substances

  • RNA
  • DNA