Fast interferometric second harmonic generation microscopy

Biomed Opt Express. 2016 Jan 8;7(2):399-408. doi: 10.1364/BOE.7.000399. eCollection 2016 Feb 1.


We report the implementation of fast Interferometric Second Harmonic Generation (I-SHG) microscopy to study the polarity of non-centrosymmetric structures in biological tissues. Using a sample quartz plate, we calibrate the spatially varying phase shift introduced by the laser scanning system. Compensating this phase shift allows us to retrieve the correct phase distribution in periodically poled lithium niobate, used as a model sample. Finally, we used fast interferometric second harmonic generation microscopy to acquire phase images in tendon. Our results show that the method exposed here, using a laser scanning system, allows to recover the polarity of collagen fibrils, similarly to standard I-SHG (using a sample scanning system), but with an imaging time about 40 times shorter.

Keywords: (170.6935) Tissue characterization; (180.4315) Nonlinear microscopy; (190.2620) Harmonic generation and mixing; (190.4180) Multiphoton processes; (190.4710) Optical nonlinearities in organic materials.