New analytical application of antibody-based biosensor in estimation of thyroid-stimulating hormone in serum

Bioanalysis. 2016 Apr;8(7):625-32. doi: 10.4155/bio-2015-0034. Epub 2016 Mar 15.

Abstract

Background: Conventionally, ELISA is used to measure thyroid-stimulating hormone (TSH) for diagnosis of thyroid disease. In this study, an immunosensor-based, kinetic-exclusion analysis (KinExA) was used for TSH estimation.

Methodology: A PMMA microbead column coated with TSH antigen is formed inside the flow cell. Samples consisting of mouse anti-TSH monoclonal antibody and TSH antigen complex in solution are passed over the beads and the unbound anti-TSH antibody is captured by the TSH-coated beads, followed by passing fluorescent-labeled antibody over the beads to generate signals for analysis. The limit of detection for the assay was 0.4 mIU l(-1) and the precision was acceptable.

Conclusion: The developed sensor was advantageous due to the automated nature and its convenience, without compromising the sensitivity for estimation of TSH.

Keywords: ELISA; TSH; biosensor; thyroid disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / immunology*
  • Biosensing Techniques* / standards
  • Calibration
  • Fluorescent Dyes / chemistry
  • Kinetics
  • Limit of Detection
  • Mice
  • Polymethyl Methacrylate / chemistry
  • Thyrotropin / blood*
  • Thyrotropin / immunology
  • Thyrotropin / standards

Substances

  • Antibodies, Monoclonal
  • Fluorescent Dyes
  • Thyrotropin
  • Polymethyl Methacrylate