Microarray expression analysis of genes involved in innate immune memory in peritoneal macrophages

Keisuke Yoshida; Claire Renard-Guillet; Kentaro Inoue; Katsuhiko Shirahige; Mariko Okada-Hatakeyama; Shunsuke Ishii

doi:10.1016/j.gdata.2015.11.028

Microarray expression analysis of genes involved in innate immune memory in peritoneal macrophages

Genom Data. 2015 Dec 1:7:90-1. doi: 10.1016/j.gdata.2015.11.028. eCollection 2016 Mar.

Authors

Keisuke Yoshida¹, Claire Renard-Guillet², Kentaro Inoue³, Katsuhiko Shirahige², Mariko Okada-Hatakeyama³, Shunsuke Ishii¹

Affiliations

¹ Laboratory of Molecular Genetics, CREST Research Project of JST (Japan Science and Technology Agency), RIKEN Tsukuba institute, Tsukuba 305-0074, Japan.
² Laboratory of Genome Structure and Function, Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 153-8904, Japan.
³ Laboratory for Integrated Cellular Systems, RIKEN Center for Integrative Medical Sciences, Yokohama 230-0045, Japan.

Abstract

Immunological memory has been believed to be a feature of the adaptive immune system for long period, but recent reports suggest that the innate immune system also exhibits memory-like reaction. Although evidence of innate immune memory is accumulating, no in vivo experimental data has clearly implicated a molecular mechanism, or even a cell-type, for this phenomenon. In this study of data deposited into Gene Expression Omnibus (GEO) under GSE71111, we analyzed the expression profile of peritoneal macrophages isolated from mice pre-administrated with toll-like receptor (TLR) ligands, mimicking pathogen infection. In these macrophages, increased expression of a group of innate immunity-related genes was sustained over a long period of time, and these genes overlapped with ATF7-regulated genes. We conclude that ATF7 plays an important role in innate immune memory in macrophages.

Keywords: ATF7; Innate immune memory; Macrophage; Microarray.