5-((3-Amidobenzyl)oxy)nicotinamides as Sirtuin 2 Inhibitors

J Med Chem. 2016 Apr 14;59(7):2928-41. doi: 10.1021/acs.jmedchem.5b01376. Epub 2016 Mar 25.


Derived from our previously reported human sirtuin 2 (SIRT2) inhibitors that were based on a 5-aminonaphthalen-1-yloxy nicotinamide core structure, 5-((3-amidobenzyl)oxy)nicotinamides offered excellent activity against SIRT2 and high isozyme selectivity over SIRT1 and SIRT3. Selected compounds also exhibited generally favorable in vitro absorption, distribution, metabolism, and excretion properties. Kinetic studies revealed that a representative SIRT2 inhibitor acted competitively against both NAD(+) and the peptide substrate, an inhibitory modality that was supported by our computational study. More importantly, two selected compounds exhibited significant protection against α-synuclein aggregation-induced cytotoxicity in SH-SY5Y cells. Therefore, 5-((3-amidobenzyl)oxy)nicotinamides represent a new class of SIRT2 inhibitors that are attractive candidates for further lead optimization in our continued effort to explore selective inhibition of SIRT2 as a potential therapy for Parkinson's disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chemistry Techniques, Synthetic
  • Dogs
  • Drug Stability
  • Humans
  • Kinetics
  • Madin Darby Canine Kidney Cells / drug effects
  • Mice
  • Microsomes, Liver / drug effects
  • Niacinamide / chemistry*
  • Sirtuin 2 / antagonists & inhibitors*
  • Sirtuins / antagonists & inhibitors
  • Structure-Activity Relationship
  • alpha-Synuclein / metabolism
  • alpha-Synuclein / toxicity


  • alpha-Synuclein
  • Niacinamide
  • SIRT2 protein, human
  • SIRT5 protein, human
  • Sirtuin 2
  • Sirtuins