High-speed detection of DNA translocation in nanopipettes

Nanoscale. 2016 Apr 14;8(14):7604-11. doi: 10.1039/c5nr08634e.

Abstract

We present a high-speed electrical detection scheme based on a custom-designed CMOS amplifier which allows the analysis of DNA translocation in glass nanopipettes on a microsecond timescale. Translocation of different DNA lengths in KCl electrolyte provides a scaling factor of the DNA translocation time equal to p = 1.22, which is different from values observed previously with nanopipettes in LiCl electrolyte or with nanopores. Based on a theoretical model involving electrophoresis, hydrodynamics and surface friction, we show that the experimentally observed range of p-values may be the result of, or at least be affected by DNA adsorption and friction between the DNA and the substrate surface.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA* / analysis
  • DNA* / chemistry
  • Lithium Chloride / chemistry
  • Models, Chemical*
  • Nanotechnology* / instrumentation
  • Nanotechnology* / methods
  • Potassium Chloride / chemistry

Substances

  • Potassium Chloride
  • DNA
  • Lithium Chloride