A calcium-dependent antibody for identification and purification of recombinant proteins

Biotechniques. 1989 Jun;7(6):580-9.


We report a straightforward methodology for purification of recombinant proteins by incorporating a short hydrophilic peptide marker segment at their N-termini. A calcium-dependent antibody that reacts primarily with the first three amino acids of this peptide segment was used to affinity purify the fusion proteins in a single chromatographic step. The marker peptide could subsequently be removed by proteolysis with the enzyme enterokinase.

MeSH terms

  • Antibodies, Monoclonal*
  • Calcium*
  • Chromatography, Affinity / methods*
  • Enteropeptidase
  • Genetic Vectors
  • Interleukin-3 / genetics
  • Interleukin-3 / isolation & purification*
  • Interleukin-4 / genetics
  • Interleukin-4 / isolation & purification*
  • Plasmids
  • Recombinant Proteins / isolation & purification*
  • Saccharomyces cerevisiae


  • Antibodies, Monoclonal
  • Interleukin-3
  • Recombinant Proteins
  • Interleukin-4
  • Enteropeptidase
  • Calcium