Placental biomarkers and angiogenic factors in oral fluids of patients with preeclampsia

Prenat Diagn. 2016 May;36(5):476-82. doi: 10.1002/pd.4811. Epub 2016 Apr 7.


Objectives: The objectives of this study are to explore the feasibility of measuring endothelial and placental biomarkers in saliva and gingival crevicular fluid (GCF) and to determine if patients with preeclampsia (PE) have a different profile of these biomarkers in oral fluids.

Method: A case-control study was conducted, including patients with PE (n = 10) and a control group with normal pregnancies randomly selected (n = 20) admitted at the Sótero del Río Hospital in Santiago, Chile. A complete periodontal and obstetric history that involved the collection of oral fluids was performed at the same gestational age. Levels of Cd63(+) extracellular vesicles, placental alkaline phosphatase (PLAP), placental growth factor (PlGF), and sFlt-1 levels were determined by ELISA assays. Data analysis was performed with chi-square or Fisher's exact test, and Mann-Whitney U-test for continuous variables. The association was assessed using a multiple logistic regression model.

Results: sFlt-1 concentrations in saliva and GCF were significantly higher in patients with PE (p = 0.045 and p = 0.033 respectively). Concentrations of PLAP were elevated in GCF of patients with PE (p = 0.049). The PLAP/CD63(+) ratio in GCF of patients with PE was significantly higher (p = 0.0008). No differences in PlGF levels were observed.

Conclusion(s): GCF of patients with PE concentrates higher levels of biomarkers related with the PE development. © 2016 John Wiley & Sons, Ltd.

MeSH terms

  • Adolescent
  • Adult
  • Alkaline Phosphatase / metabolism*
  • Biomarkers / metabolism
  • Body Fluids / chemistry
  • Case-Control Studies
  • Chile
  • Enzyme-Linked Immunosorbent Assay
  • Extracellular Vesicles / metabolism*
  • Female
  • GPI-Linked Proteins / metabolism
  • Gingiva
  • Humans
  • Isoenzymes / metabolism*
  • Logistic Models
  • Multivariate Analysis
  • Placenta Growth Factor / metabolism*
  • Pre-Eclampsia / metabolism*
  • Pregnancy
  • Saliva / chemistry*
  • Tetraspanin 30 / metabolism*
  • Vascular Endothelial Growth Factor Receptor-1 / metabolism*
  • Young Adult


  • Biomarkers
  • CD63 protein, human
  • GPI-Linked Proteins
  • Isoenzymes
  • PGF protein, human
  • Tetraspanin 30
  • Placenta Growth Factor
  • FLT1 protein, human
  • Vascular Endothelial Growth Factor Receptor-1
  • Alkaline Phosphatase
  • alkaline phosphatase, placental