Characterization of Arabidopsis thaliana FLAVONOL SYNTHASE 1 (FLS1) -overexpression plants in response to abiotic stress

Plant Physiol Biochem. 2016 Jun;103:133-42. doi: 10.1016/j.plaphy.2016.03.010. Epub 2016 Mar 9.

Abstract

Flavonoids are an important group of secondary metabolites that are involved in plant growth and contribute to human health. Many studies have focused on the biosynthesis pathway, biochemical characters, and biological functions of flavonoids. In this report, we showed that overexpression of FLS1 (FLS1-OX) not only altered seed coat color (resulting in a light brown color), but also affected flavonoid accumulation. Whereas fls1-3 mutants accumulated higher anthocyanin levels, FLS1-OX seedlings had lower levels than those of the wild-type. Besides, shoot tissues of FLS1-OX plants exhibited lower flavonol levels than those of the wild-type. However, growth performance and abiotic stress tolerance of FLS1-OX, fls1-3, and wild-type plants were not significantly different. Taken together, FLS1 can be manipulated (i.e., silenced or overexpressed) to redirect the flavonoid biosynthetic pathway toward anthocyanin production without negative effects on plant growth and development.

Keywords: Abiotic stress; Abscisic acid (ABA); Anthocyanin; Arabidopsis thaliana; FLS1; Flavonol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / metabolism
  • Anthocyanins / metabolism
  • Arabidopsis / cytology
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Arabidopsis / physiology
  • Biosynthetic Pathways
  • Flavonoids / metabolism*
  • Flowers / cytology
  • Flowers / enzymology
  • Flowers / genetics
  • Flowers / physiology
  • Gene Expression
  • Gene Expression Regulation, Plant*
  • Genes, Reporter
  • Oxidoreductases / genetics
  • Oxidoreductases / metabolism*
  • Plant Growth Regulators / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plants, Genetically Modified
  • Seedlings / cytology
  • Seedlings / enzymology
  • Seedlings / genetics
  • Seedlings / physiology
  • Seeds / cytology
  • Seeds / enzymology
  • Seeds / genetics
  • Seeds / physiology
  • Stress, Physiological

Substances

  • Anthocyanins
  • Flavonoids
  • Plant Growth Regulators
  • Plant Proteins
  • Abscisic Acid
  • Oxidoreductases
  • flavonol synthase