Color-Coded Super-Resolution Small-Molecule Imaging

Chembiochem. 2016 Jun 2;17(11):999-1003. doi: 10.1002/cbic.201600013. Epub 2016 Apr 26.


Although the development of super-resolution microscopy dates back to 1994, its applications have been primarily focused on visualizing cellular structures and targets, including proteins, DNA and sugars. We now report on a system that allows both monitoring of the localization of exogenous small molecules in live cells at low resolution and subsequent super-resolution imaging by using stochastic optical reconstruction microscopy (STORM) on fixed cells. This represents a powerful new tool to understand the dynamics of subcellular trafficking associated with the mode and mechanism of action of exogenous small molecules.

Keywords: drug discovery; microscopy; mode of action; natural products; super-resolution imaging.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / metabolism
  • Carbocyanines / chemistry
  • Cell Line, Tumor
  • Color
  • DNA / chemistry
  • DNA / metabolism
  • Humans
  • Microscopy, Fluorescence*
  • Proteins / chemistry*
  • Proteins / metabolism
  • Small Molecule Libraries / chemistry*


  • Alexa Fluor 647
  • Antibodies, Monoclonal
  • Carbocyanines
  • Proteins
  • Small Molecule Libraries
  • DNA