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, 11 (3), e0150882
eCollection

The Role of Lumbar Sympathetic Nerves in Regulation of Blood Flow to Skeletal Muscle During Anaphylactic Hypotension in Anesthetized Rats

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The Role of Lumbar Sympathetic Nerves in Regulation of Blood Flow to Skeletal Muscle During Anaphylactic Hypotension in Anesthetized Rats

Jie Song et al. PLoS One.

Abstract

During hypovolemic shock, skeletal muscle blood flow could be redistributed to vital organs via vasoconstriction in part evoked by activation of the innervating sympathetic nerve activity. However, it is not well known whether this mechanism operates during anaphylactic shock. We determined the femoral artery blood flow (FBF) and lumbar sympathetic nerve activity (LSNA) mainly regulating the hindquater muscle blood flow during anaphylactic hypotension in anesthetized rats. Anesthetized Sprague-Dawley rats were randomly allocated to the following groups (n = 7/group): (1) non-sensitized, (2) anaphylaxis, (3) anaphylaxis-lumbar sympathectomy (LS) and (4) anaphylaxis-sinoaortic denervation (SAD) groups. Anaphylaxis was induced by an intravenous injection of the ovalbumin antigen to the sensitized rats. The systemic arterial pressure (SAP), heart rate (HR), central venous pressure (CVP), FBF and LSNA were continuously measured. In the anaphylaxis group, LSNA and HR increased, while SAP and FBF decreased after antigen injection. In the anaphylaxis-SAD group, LSNA did not significantly change during the early phase, but the responses of SAP and FBF were similar to those in the anaphylaxis group. In the anaphylaxis-LS group, both FBF and SAP decreased similarly to the anaphylaxis group during anaphylactic hypotension. These results indicated that LSNA increased via baroreceptor reflex, but this sympathoexcitation or LS did not affect antigen-induced decreases in FBF or SAP. Lumbar sympathetic nerves are not involved in regulation of the blood flow to the hindlimb or systemic blood pressure during anaphylactic hypotension in anesthetized rats.

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1
Representative recordings of the sympathetic and hemodynamic responses after an intravenous injection of the SNP or the ovalbumin antigen in a non-sensitized (A; SNP, B; ovalbumin antigen), an anaphylaxis rat (C; SNP, D; ovalbumin antigen) and an anaphylaxis rat with SAD (E; SNP, F; ovalbumin antigen). LSNA, lumbar sympathetic nerve activity; HR, heart rate; SAP, systemic arterial pressure; FBF, femoral arterial blood flow; CVP, central venous pressure; FVR, femoral vascular resistance.
Fig 2
Fig 2. Summary of the LSNA, FBF and FVR changes during anaphylaxis.
The time-course data of LSNA (A), FBF (B) and FVR (C) after an intravenous injection of the ovalbumin antigen were shown. ● non-sensitized group; ○ anaphylaxis group; ◇ anaphylaxis-LS group; △ anaphylaxis-SAD group. n = 7 rats per group in this experiment. Values are expressed as means±SE. #P<0.05 vs. the non-sensitized group. *P<0.05 vs. the anaphylaxis group. The black, white and gray bars show the significant differences from the corresponding baseline values in the anaphylaxis group, anaphylaxis-LS group and anaphylaxis-SAD group, respectively.
Fig 3
Fig 3. Summary of the hemodynamic changes during anaphylaxis.
The time-course data of the SAP (A) and HR (B) after an intravenous injection of the ovalbumin antigen were shown. ● non-sensitized group; ○ anaphylaxis group; ◇ anaphylaxis-LS group; △ anaphylaxis-SAD group. n = 7 rats per group in this experiment. Values are expressed as means±SE. #P<0.05 vs. the non-sensitized group. *P<0.05 vs. the anaphylaxis group. The black, white and gray bars show the significant differences from the corresponding baseline values in the anaphylaxis group, anaphylaxis-LS group and anaphylaxis-SAD group, respectively.

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References

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Publication types

Grant support

This study is supported by grants from Grant for Promoted Research from Kanazawa Medical University (MT, S2013-1, http://www.kanazawa-med.ac.jp/kenkyu/publication.html), from Mishima Kaiun Memorial Foundation (MT, No11, http://www.mishima-kaiun.or.jp/assist/2147.html) and from Takeda Science Foundation (MT, http://www.takeda-sci.or.jp/business/doc/2013_list.pdf). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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