Somatic CPEB4 and CPEB1 genes mutations spectrum on the prognostic predictive accuracy in patients with high-grade glioma and their clinical significance

J Neurol Sci. 2016 Apr 15:363:80-3. doi: 10.1016/j.jns.2016.02.032. Epub 2016 Feb 16.

Abstract

Background: Glioblastoma (grade IV glioma/GBM) is characterized by extremely aggressive invasion and proliferative nature.

Objective: The main goal of this study was to evaluate the expression patterns of CPEB1 and CPEB4 in glioma patients.

Methods: 41 paraffin-embedded tissue samples with glioma (WHO I-IV) were collected between January 2008 and December 2012 in Tehran, Iran. MRI of patients was done before and within 24 h after surgery and gliomas investigated using quantitative real-time PCR and immunohistochemistry. Kaplan-Meier survival and Cox regression were applied to assess the prognosis of patients.

Results: The mRNA level of CPEB4 was strongly increased in tumor tissues (0.67±3.154 vs. 1.671±0.51; P=0.001). Furthermore, CPEB1 mRNA was significantly decreased in tumor tissues compared to normal tissues (2.852±0.587 vs. 1.471±0.862; P=0.025). Our findings showed that CPEB4 levels was markedly increased in patients with advanced grade gliomas (P=0.003). In addition, CPEB1 mRNA levels were not associated with clinicopathological features. Of the 41 cases, high CPEB4 expression was found in 29 patients (70.73%), while 12 cases (29.26%) showed weak expression levels, while the protein expression of CPEB4 were remarkably weak in normal tissues (P=0.001). However, no correlation was found between expression levels of CPEB1 and clinicopathological characteristics. Kaplan-Meier survival and log-rank test indicated that high expression of CPEB4 was correlated with shorter overall survival (log-rank test P<0.001). Furthermore, low expression of CPEB1 was linked to shorter overall survival (log-rank test P=0.021). Multivariate Cox proportional hazards model showed that high CPEB1 (P=0.027), low CPEB4 expressions (P=0.021), and advanced tumor grade (P=0.036) were independent predictor of overall survival.

Conclusion: Our data indicated expressions levels of CPEB4 and CPEB1 are correlated with overall survival in patients with glioma.

Keywords: Analysis; Biomarker; Glioma; IHC; PCR; Patient; Survival.

MeSH terms

  • Adult
  • Biomarkers, Tumor / genetics*
  • Brain Neoplasms / diagnosis
  • Brain Neoplasms / genetics*
  • Brain Neoplasms / mortality
  • Female
  • Follow-Up Studies
  • Glioma / diagnosis
  • Glioma / genetics*
  • Glioma / mortality
  • Humans
  • Male
  • Middle Aged
  • Mutation / genetics*
  • Predictive Value of Tests
  • Prognosis
  • RNA-Binding Proteins / genetics*
  • Survival Rate / trends
  • Transcription Factors / genetics*
  • mRNA Cleavage and Polyadenylation Factors / genetics*

Substances

  • Biomarkers, Tumor
  • CPEB1 protein, human
  • CPEB4 protein, human
  • RNA-Binding Proteins
  • Transcription Factors
  • mRNA Cleavage and Polyadenylation Factors