Advanced Molecular Detection of Malarone Resistance

Eldin Talundzic; Mateusz M Plucinski; Shweta Biliya; Luciana M Silva-Flannery; Paul M Arguin; Eric S Halsey; John W Barnwell; Fredrik Vannberg; Venkatachalam Udhayakumar

doi:10.1128/AAC.00171-16

Advanced Molecular Detection of Malarone Resistance

Antimicrob Agents Chemother. 2016 May 23;60(6):3821-3. doi: 10.1128/AAC.00171-16. Print 2016 Jun.

Authors

Eldin Talundzic¹, Mateusz M Plucinski², Shweta Biliya³, Luciana M Silva-Flannery⁴, Paul M Arguin⁵, Eric S Halsey², John W Barnwell⁵, Fredrik Vannberg³, Venkatachalam Udhayakumar⁵

Affiliations

¹ Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, USA Atlanta Research and Education Foundation, Veterans Affairs Medical Center, Atlanta, Georgia, USA etalundzic@cdc.gov.
² Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, USA President's Malaria Initiative, Atlanta, Georgia, USA.
³ School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA.
⁴ Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, USA Atlanta Research and Education Foundation, Veterans Affairs Medical Center, Atlanta, Georgia, USA.
⁵ Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Abstract

The rapid emergence of drug-resistant malaria parasites during the course of an infection remains a major challenge for providing accurate treatment guidelines. This is particularly important in cases of malaria treatment failure. Using a previously well-characterized case of malaria treatment failure, we show the utility of using next-generation sequencing for early detection of the rise and selection of a previously reported atovaquone-proguanil (malarone) drug resistance-associated mutation.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antimalarials / therapeutic use
Atovaquone / therapeutic use
Cytochromes b / genetics*
Drug Combinations
Drug Resistance / genetics*
Gene Expression
High-Throughput Nucleotide Sequencing
Humans
Malaria, Falciparum / drug therapy
Malaria, Falciparum / parasitology
Male
Mutation*
Nigeria
Plasmodium falciparum / drug effects
Plasmodium falciparum / genetics*
Plasmodium falciparum / isolation & purification
Proguanil / therapeutic use
Protozoan Proteins / genetics*
Travel
Treatment Failure
United States

Substances

Antimalarials
Drug Combinations
Protozoan Proteins
atovaquone, proguanil drug combination
Cytochromes b
Proguanil
Atovaquone

Grants and funding

We acknowledge support from the Advanced Molecular Detection Initiative at the CDC. E.T. is supported by the Atlanta Research and Education Foundation.