Evaluation of Protective Effects of Bioactive Phytochemicals Against Methotrexate in Salmonella typhimurium TA1535/pSK1002 Coupled with Micronucleus Assay

Biomed Environ Sci. 2016 Feb;29(2):148-52. doi: 10.3967/bes2016.017.

Abstract

We evaluated the antimutagenic effects of 10 kinds of bioactive phytochemicals and some phytochemical combinations against methotrexate (MTX)-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/pSK1002 combined with a micronucleus assay. We observed that allicin, proanthocyanidins, polyphenols, eleutherosides, and isoflavones had higher antimutagenic activities than the other five types of bioactive phytochemicals. At the highest dose tested, MTX-induced genotoxicity was inhibited by 25%-75%. Kunming mice treated by MTX along with bioactive phytochemical combinations showed significant reduction in micronucleus induction and sperm abnormality rate (P<0.01). These results indicate that bioactive phytochemical combinations can be potentially used as new cytoprotectors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antimetabolites, Antineoplastic / adverse effects*
  • Cytoprotection*
  • Drug Evaluation, Preclinical
  • Female
  • Male
  • Methotrexate / adverse effects*
  • Mice
  • Micronucleus Tests
  • Phytotherapy*
  • Plant Extracts*
  • Random Allocation
  • Salmonella typhimurium

Substances

  • Antimetabolites, Antineoplastic
  • Plant Extracts
  • Methotrexate