Vascular Influence on Ventral Telencephalic Progenitors and Neocortical Interneuron Production

Dev Cell. 2016 Mar 21;36(6):624-38. doi: 10.1016/j.devcel.2016.02.023.

Abstract

The neocortex contains glutamatergic excitatory neurons and γ-aminobutyric acid (GABA)ergic inhibitory interneurons. Extensive studies have revealed substantial insights into excitatory neuron production. However, our knowledge of the generation of GABAergic interneurons remains limited. Here we show that periventricular blood vessels selectively influence neocortical interneuron progenitor behavior and neurogenesis. Distinct from those in the dorsal telencephalon, radial glial progenitors (RGPs) in the ventral telencephalon responsible for producing neocortical interneurons progressively grow radial glial fibers anchored to periventricular vessels. This progenitor-vessel association is robust and actively maintained as RGPs undergo interkinetic nuclear migration and divide at the ventricular zone surface. Disruption of this association by selective removal of INTEGRIN β1 in RGPs leads to a decrease in progenitor division, a loss of PARVALBUMIN and SOMATOSTATIN-expressing interneurons, and defective synaptic inhibition in the neocortex. These results highlight a prominent interaction between RGPs and periventricular vessels important for proper production and function of neocortical interneurons.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / metabolism
  • Female
  • Gestational Age
  • Green Fluorescent Proteins / metabolism
  • Integrin beta1 / metabolism
  • Interneurons / cytology*
  • Interneurons / metabolism
  • Median Eminence / blood supply
  • Median Eminence / embryology
  • Median Eminence / metabolism
  • Mice
  • Mice, Transgenic
  • Neocortex / blood supply*
  • Neocortex / embryology*
  • Neocortex / metabolism
  • Neural Stem Cells / cytology*
  • Neural Stem Cells / metabolism
  • Neuroglia / cytology
  • Neuroglia / metabolism
  • Parvalbumins / metabolism
  • Pregnancy
  • Preoptic Area / blood supply
  • Preoptic Area / embryology
  • Preoptic Area / metabolism
  • Recombinant Proteins / metabolism
  • Somatostatin / metabolism
  • Telencephalon / blood supply*
  • Telencephalon / embryology*
  • Telencephalon / metabolism

Substances

  • Integrin beta1
  • Parvalbumins
  • Recombinant Proteins
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • Somatostatin