Lipidomic profiling of dried seahorses by hydrophilic interaction chromatography coupled to mass spectrometry

Food Chem. 2016 Aug 15:205:89-96. doi: 10.1016/j.foodchem.2016.02.151. Epub 2016 Mar 3.

Abstract

Dried seahorse is a precious raw food material for cooking soups. In this study, a lipidomics strategy using the techniques of solid-phase extraction (SPE) and hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-QTOF/MS) was developed for extraction, visualization, and quantification of phospholipids in dried seahorses. The parameters of SPE were optimized, and 1 mL of sample and chloroform/methanol (1:2, v/v) were found to be the best loading volume and eluting solvent, respectively. Afterwards, each phospholipid class was successfully separated on a HILIC column and analyzed by mass spectrometry. A total of 50 phospholipid molecular species were identified and determined, including 15 phosphatidylcholines (PCs), 14 phosphatidylethanolamines (PEs), 12 phosphatidylinositols (PIs) and 9 phosphatidylserines (PSs). In comparison to previously methods, this strategy was robust and efficient in extraction, characterization, and determination of phospholipids. The dried seahorse was found to contain large amounts of polyunsaturated fatty acyl phospholipids which are beneficial to human health.

Keywords: Hydrophilic interaction chromatography; Lipidomics; Quadruple/time-of-flight mass spectrometry; Seahorse; Solid-phase extraction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Liquid / methods
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Phosphatidylcholines / analysis
  • Phosphatidylcholines / chemistry
  • Phosphatidylethanolamines / analysis
  • Phospholipids / analysis*
  • Phospholipids / chemistry
  • Seafood / analysis*
  • Smegmamorpha*
  • Solid Phase Extraction / methods
  • Tandem Mass Spectrometry / methods

Substances

  • Phosphatidylcholines
  • Phosphatidylethanolamines
  • Phospholipids