Anti-inflammatory Effect of Mesenchymal Stromal Cell Transplantation and Quercetin Treatment in a Rat Model of Experimental Cerebral Ischemia

Cell Mol Neurobiol. 2016 Oct;36(7):1023-34. doi: 10.1007/s10571-015-0291-6. Epub 2016 Mar 23.

Abstract

Here, we have investigated the synergistic effect of quercetin administration and transplantation of human umbilical cord mesenchymal stromal cells (HUMSCs) following middle cerebral artery occlusion in rat. Combining quercetin treatment with delayed transplantation of HUMSCs after local cerebral ischemia significantly (i) improved neurological functional recovery; (ii) reduced proinflammatory cytokines (interleukin(IL)-1β and IL-6), increased anti-inflammatory cytokines (IL-4, IL-10, and transforming growth factor-β1), and reduced ED-1 positive areas; (iii) inhibited cell apoptosis (caspase-3 expression); and (iv) improved the survival rate of HUMSCs in the injury site. Altogether, our results demonstrate that combined HUMSC transplantation and quercetin treatment is a potential strategy for reducing secondary damage and promoting functional recovery following cerebral ischemia.

Keywords: Apoptosis; Inflammation; Mesenchymal stromal cells; Quercetin; Stroke.

MeSH terms

  • Animals
  • Brain Ischemia / metabolism
  • Brain Ischemia / therapy*
  • Cytokines / metabolism*
  • Disease Models, Animal
  • Female
  • Humans
  • Interleukin-10 / metabolism
  • Interleukin-4 / metabolism
  • Interleukin-6 / metabolism
  • Mesenchymal Stem Cell Transplantation* / methods
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / immunology
  • Quercetin / pharmacology*
  • Rats, Sprague-Dawley
  • Umbilical Cord / cytology*

Substances

  • Cytokines
  • Interleukin-6
  • Interleukin-10
  • Interleukin-4
  • Quercetin