Molecular basis of 5-hydroxytryptophan synthesis in Saccharomyces cerevisiae

Jiantao Zhang; Chaochen Wu; Jiayuan Sheng; Xueyang Feng

doi:10.1039/c5mb00888c

Molecular basis of 5-hydroxytryptophan synthesis in Saccharomyces cerevisiae

Mol Biosyst. 2016 Apr 26;12(5):1432-5. doi: 10.1039/c5mb00888c.

Authors

Jiantao Zhang¹, Chaochen Wu², Jiayuan Sheng², Xueyang Feng²

Affiliations

¹ Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. xueyang@vt.edu and Department of Pharmacology & Toxicology, University of Arizona, Tucson, AZ 85721, USA.
² Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. xueyang@vt.edu.

PMID: 27008988
DOI: 10.1039/c5mb00888c

Abstract

We report for the first time that 5-hydroxytryptophan can be synthesized in Saccharomyces cerevisiae by heterologously expressing prokaryotic phenylalanine 4-hydroxylase or eukaryotic tryptophan 3/5-hydroxylase, together with enhanced synthesis of MH4 or BH4 cofactors. The innate DFR1 gene in the folate synthesis pathway was found to play pivotal roles in 5-hydroxytryptophan synthesis.

Publication types

Review

MeSH terms

5-Hydroxytryptophan / biosynthesis*
Biopterins / analogs & derivatives
Biopterins / metabolism
Folic Acid / biosynthesis
Gene Expression Regulation, Fungal
Phenylalanine Hydroxylase / genetics
Phenylalanine Hydroxylase / metabolism
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*
Tryptophan Hydroxylase / genetics
Tryptophan Hydroxylase / metabolism

Substances

Biopterins
Folic Acid
5-Hydroxytryptophan
Phenylalanine Hydroxylase
Tryptophan Hydroxylase
sapropterin