Matrix Gla protein repression by miR-155 promotes oncogenic signals in breast cancer MCF-7 cells

FEBS Lett. 2016 Apr;590(8):1234-41. doi: 10.1002/1873-3468.12155. Epub 2016 Apr 6.

Abstract

MGP is a protein that was initially associated with the inhibition of calcification in skeleton, soft tissues, and arteries, but more recently also implicated in cancer. In breast cancer, higher levels of MGP mRNA were associated with poor prognosis, but since this deregulation was never demonstrated at the protein level, we postulated the involvement of a post-transcriptional regulatory mechanism. In this work we show that MGP is significantly repressed by miR-155 in breast cancer MCF-7 cells, and concomitantly there is a stimulation of cell proliferation and cell invasiveness. This study brings new insights into the putative involvement of MGP and oncomiR-155 in breast cancer, and may contribute to develop new therapeutic strategies.

Keywords: MCF-7 cells; MGP; breast cancer; miR-155; oncogenesis.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Calcium-Binding Proteins / genetics*
  • Calcium-Binding Proteins / metabolism
  • Carcinogenesis / genetics*
  • Cell Proliferation
  • Extracellular Matrix Proteins / genetics*
  • Extracellular Matrix Proteins / metabolism
  • Female
  • Gene Expression Regulation, Neoplastic
  • Gene Silencing
  • HEK293 Cells
  • Humans
  • MCF-7 Cells
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Models, Biological
  • Neoplasm Invasiveness
  • RNA, Small Interfering / metabolism
  • Signal Transduction*

Substances

  • Calcium-Binding Proteins
  • Extracellular Matrix Proteins
  • MIRN155 microRNA, human
  • MicroRNAs
  • RNA, Small Interfering
  • matrix Gla protein

Associated data

  • GENBANK/NM_000900.3