PAX3+ skeletal muscle satellite cells retain long-term self-renewal and proliferation

Muscle Nerve. 2016 Nov;54(5):943-951. doi: 10.1002/mus.25117. Epub 2016 Jun 13.

Abstract

Introduction: Different populations of satellite cells (SCs) have been identified, but their functional difference remains unclear.

Methods: We used cell-surface markers and paired box transcription factor 3 (Pax3)/paired box transcription factor 7 (Pax7) expression to separate SC populations. In addition, self-renewal, proliferation, and differentiation abilities of each population were analyzed.

Results: Pax3+ /Pax7- SCs exhibited higher proliferation ability characterized by forming clusters of myogenic colonies with more self-renewing cells after several passages, while Pax3- /Pax7+ SCs had faster differentiation. The myotubes derived from Pax3+ /Pax7- SCs tended to express slow-myosin heavy chain and exhibited rhythmic contraction, while myotubes originating from Pax3- /Pax7+ SCs primarily formed fast-myosin heavy chains characterized by transitory contraction.

Conclusions: Pax3+ /Pax7- SCs exhibited the ability of long-term self-renewal and proliferation, whereas Pax3- /Pax7+ SCs demonstrated faster differentiation. Muscle Nerve 54: 943-951, 2016.

Keywords: Pax3; Pax7; differentiation; proliferation; satellite cells; self-renewal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Antigens, Ly / metabolism
  • Cell Differentiation / physiology*
  • Cells, Cultured
  • Disease Models, Animal
  • Embryo, Mammalian
  • Gene Expression Regulation, Developmental / physiology
  • Leukocyte Common Antigens / metabolism
  • Membrane Proteins / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Muscle Development / physiology*
  • Muscular Diseases / pathology
  • Muscular Diseases / physiopathology
  • MyoD Protein / metabolism
  • Myosin Heavy Chains / metabolism
  • PAX3 Transcription Factor / metabolism*
  • PAX7 Transcription Factor / metabolism
  • Receptors, CXCR4 / metabolism
  • Satellite Cells, Skeletal Muscle / physiology*

Substances

  • Antigens, Ly
  • CXCR4 protein, mouse
  • Ly6a protein, mouse
  • Membrane Proteins
  • MyoD Protein
  • PAX3 Transcription Factor
  • PAX7 Transcription Factor
  • Receptors, CXCR4
  • Pax3 protein, mouse
  • Leukocyte Common Antigens
  • Myosin Heavy Chains