Genomic, pathogenic, and antigenic comparisons of Getah virus strains isolated in 1978 and 2014 in Japan

Arch Virol. 2016 Jun;161(6):1691-5. doi: 10.1007/s00705-016-2840-9. Epub 2016 Mar 26.

Abstract

A Getah virus strain isolated during an outbreak in racehorses in Japan in 2014 (14-I-605) was compared with the vaccine strain isolated in 1978 (MI-110). A comparison of the genome sequences of these strains revealed seven amino acid substitutions in non-structural protein 3, and one or two substitutions in each of other non-structural proteins. In contrast, the structural proteins were highly conserved (99.8-99.9 % amino acid sequence identity). Horse antisera raised against the MI-110 strain showed similar virus-neutralization titers against both MI-110 and 14-I-605 strains (512 and 256, respectively). Therefore, antigenic mutation was probably not a direct cause of the outbreak that occurred in 2014.

Publication types

  • Comparative Study

MeSH terms

  • Alphavirus / genetics*
  • Alphavirus / immunology
  • Alphavirus / pathogenicity
  • Alphavirus Infections / epidemiology
  • Alphavirus Infections / veterinary*
  • Alphavirus Infections / virology
  • Amino Acid Substitution
  • Animals
  • Antibodies, Neutralizing / blood
  • Antibodies, Viral / blood
  • Antigens, Viral / genetics
  • Cross Reactions
  • Disease Outbreaks / veterinary
  • Genome, Viral
  • Horse Diseases / epidemiology
  • Horse Diseases / virology*
  • Horses
  • Japan / epidemiology
  • Phylogeny
  • Time Factors
  • Viral Proteins / genetics
  • Viral Proteins / immunology

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Antigens, Viral
  • Viral Proteins