Lipase cocktail for efficient conversion of oils containing phospholipids to biodiesel

Bioresour Technol. 2016 Jul;211:224-30. doi: 10.1016/j.biortech.2016.03.062. Epub 2016 Mar 14.


The presence of phospholipid has been a challenge in liquid enzymatic biodiesel production. Among six lipases that were screened, lipase AY had the highest hydrolysis activity and a competitive transesterification activity. However, it yielded only 21.1% FAME from oil containing phospholipids. By replacing portions of these lipases with a more robust bioFAME lipase, CalT, the combination of lipase AY-CalT gave the highest FAME yield with the least amounts of free fatty acids and partial glycerides. A higher methanol addition rate reduced FAME yields for lipase DF-CalT and A10D-CalT combinations while that of lipase AY-CalT combination improved. Optimizing the methanol addition rate for lipase AY-CalT resulted in a FAME yield of 88.1% at 2h and more than 95% at 6h. This effective use of lipases could be applied for the rapid and economic conversion of unrefined oils to biodiesel.

Keywords: Biodiesel; Enzyme biocatalysis; Mixed lipase; Phospholipid; Transesterification.

MeSH terms

  • Biofuels*
  • Hydrolysis
  • Lipase / chemistry*
  • Oils / chemistry*
  • Phospholipids / chemistry*


  • Biofuels
  • Oils
  • Phospholipids
  • Lipase