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Identification of a New Hominin Bone From Denisova Cave, Siberia Using Collagen Fingerprinting and Mitochondrial DNA Analysis


Identification of a New Hominin Bone From Denisova Cave, Siberia Using Collagen Fingerprinting and Mitochondrial DNA Analysis

Samantha Brown et al. Sci Rep.


DNA sequencing has revolutionised our understanding of archaic humans during the Middle and Upper Palaeolithic. Unfortunately, while many Palaeolithic sites contain large numbers of bones, the majority of these lack the diagnostic features necessary for traditional morphological identification. As a result the recovery of Pleistocene-age human remains is extremely rare. To circumvent this problem we have applied a method of collagen fingerprinting to more than 2000 fragmented bones from the site of Denisova Cave, Russia, in order to facilitate the discovery of human remains. As a result of our analysis a single hominin bone (Denisova 11) was identified, supported through in-depth peptide sequencing analysis, and found to carry mitochondrial DNA of the Neandertal type. Subsequent radiocarbon dating revealed the bone to be >50,000 years old. Here we demonstrate the huge potential collagen fingerprinting has for identifying hominin remains in highly fragmentary archaeological assemblages, improving the resources available for wider studies into human evolution.


Figure 1
Figure 1. MALDI-ToF mass spectrum of digested collagen from DC1227.
Previously published human markers are labelled A–G. All numbered peaks represent confirmed sequencing-matched peptides observed in human collagen (except E which is only known through similarity to homologous markers in other species9).
Figure 2
Figure 2. Photograph of DC1227, detailing each visible surface of the bone.
Figure 3
Figure 3. Micro-CT Scan of DC1227, (a,b) surface of DC1227, (c) projection through the length of DC1227, (d,e) projections through the width of DC1227.
Figure 4
Figure 4. Neighbor-joining tree relating the DC1227 mtDNA to other ancient and present-day mtDNAs.
A chimpanzee mtDNA was used as an outgroup (not shown). Support for each branch is based on 500 bootstrap replications. See Table S2 for the geographic origin of the ancient specimens.

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    1. Bolikhovskaya N. S. & Shunkov M. V. Pleistocene environments of northwestern Altai: Vegetation and climate. Arch. Ethno. Anthr. Eurasia 42, 2–17 (2014).
    1. Agadjanian A. K. The dynamics of bioresources and activity of the Palaeolithic man, using the example of northwestern altai mountains. Palaeontolog. J. 40, S482–S493 (2005).
    1. Krause J. et al. The complete mitochondrial DNA genome of an unknown hominin from southern Siberia. Nature 464, 894–897 (2010). - PubMed
    1. Reich D. et al. Genetic history of an archaic hominin group from Denisova cave in Siberia. Nature 468, 1053–1060 (2010). - PMC - PubMed
    1. Prüfer K. et al. The complete genome sequence of a Neanderthal from the Altai Mountains. Nature 505(7481), 43–49 (2014). - PMC - PubMed

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