Effects of 3-deazaadenosine, an inducer of HL-60 cell differentiation, on human blood cells in vitro

Pediatr Hematol Oncol. 1989;6(2):173-9. doi: 10.3109/08880018909034283.

Abstract

The adenosine analogue 3-deazaadenosine (c3 Ado) induces differentiation of human promyelocytic leukemia HL-60 cells in vitro. c3 Ado affects in vitro viability and growth of cells involved indirectly (cytokine-producing cells) and directly (transformed myeloid cells) in leukemic cell differentiation. Growth inhibition of proliferating cells is achieved at c3 Ado concentrations between 10-100 microM. Concentrations higher than 100 microM affect viability of proliferating and nonproliferating cells.

Publication types

  • Comparative Study

MeSH terms

  • Cell Differentiation / drug effects*
  • Cell Division
  • Cell Line
  • Concanavalin A / pharmacology
  • Dose-Response Relationship, Drug
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / drug effects*
  • Humans
  • Leukemia, Promyelocytic, Acute / pathology
  • Lymphocytes / cytology
  • Lymphocytes / drug effects*
  • Neoplastic Stem Cells / drug effects
  • Neoplastic Stem Cells / pathology
  • Stimulation, Chemical
  • Tubercidin / pharmacology*
  • Tumor Cells, Cultured / drug effects

Substances

  • 3-deazaadenosine
  • Concanavalin A
  • Tubercidin