Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation

Transpl Immunol. 2016 May;36:25-31. doi: 10.1016/j.trim.2016.03.003. Epub 2016 Mar 25.

Abstract

Background: Heat shock proteins (HSP) are highly conserved immunogenic proteins serving as potent danger signals. They are upregulated under stress conditions like fever and hypoxia. Extracellular HSP are involved in antigen presentation, cytokine release and maturation of antigen presenting cells.

Methods: The release of the inducible members of the HSP70 family, Hsp72 and Hsp70B', into the serum of 20 patients undergoing allogeneic hematopoietic cell transplantation and 20 healthy donors was evaluated using enzyme linked immunosorbent assay (ELISA) kits.

Results: Eight patients (40%) did not receive anti-thymocyte globulin (ATG) for prophylaxis of graft versus host disease (GvHD). These patients had no detectable or low serum levels of Hsp72 (n=3, 0.03 to 1.92ng/ml) which were in line with levels detected in 20 healthy individuals (p=0.07). Measurable HSP was not associated with any medication or transplantation-related procedures. In twelve patients (60%) receiving ATG, detected high levels of HSP reflected cross-reactivity of the rabbit-derived ATG with the anti-rabbit antibody used in the ELISA.

Conclusions: Assumed HSP70 expression detected such ELISA has to be regarded carefully after ATG application. Neither radiochemotherapy, nor inflammation or sepsis during aplasia induced HSP70 release into the serum. Thus, soluble HSP70 may not be involved in the pathogenesis of acute GvHD.

Keywords: Allogeneic hematopoietic cell transplantation; Anti-thymocyte globulin; Graft versus host disease; Heat shock protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Animals
  • Biomarkers / blood*
  • Cross Reactions
  • Female
  • Graft vs Host Disease / diagnosis*
  • HSP70 Heat-Shock Proteins / blood*
  • HSP72 Heat-Shock Proteins / blood*
  • Hematopoietic Stem Cell Transplantation*
  • Humans
  • Male
  • Middle Aged
  • Rabbits
  • Transplantation, Homologous

Substances

  • Biomarkers
  • HSP70 Heat-Shock Proteins
  • HSP72 Heat-Shock Proteins
  • Hsp70B' protein, human