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, 2016, 7214087

Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses

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Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses

Deepak M Kasote et al. Int J Anal Chem.

Abstract

Plant hormones are the key regulators of adaptive stress response. Abiotic stresses such as drought and salt are known to affect the growth and productivity of plants. It is well known that the levels of plant hormones such as zeatin (ZA), abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), and brassinolide (BR) fluctuate upon abiotic stress exposure. At present, there is not any single suitable liquid chromatography-mass spectrometry (LC-MS) method for simultaneous analysis of BR and other plant hormones involved in abiotic stresses. In the present study, we developed a simple, sensitive, and rapid method for simultaneous analysis of five major plant hormones, ZA, ABA, JA, SA, and BR, which are directly or indirectly involved in drought and salt stresses. The optimized extraction procedure was simple and easy to use for simultaneous measurement of these plant hormones in Arabidopsis thaliana. The developed method is highly reproducible and can be adapted for simultaneous measurement of changes in plant hormones (ZA, ABA, JA, SA, and BR) in response to abiotic stresses in plants like A. thaliana and tomato.

Figures

Figure 1
Figure 1
Structures of five plant hormones.
Figure 2
Figure 2
Typical MRM chromatograms of working solution of standard plant hormones [zeatin (ZA), abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), and brassinolide (BR)].
Figure 3
Figure 3
Typical MRM chromatograms of zeatin (ZA), abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), and brassinolide (BR) in the crude extract of A. thaliana rosette leaves.
Figure 4
Figure 4
Effect of solvents on plant hormone extraction. Concentration of endogenous plant hormones (ZA, ABA, SA, JA, and BR) which were extracted by eight different solvents [P-100% isopropanol, M-100% methanol, PW (75 : 25, isopropanol : water), MW (75 : 25, methanol : water), PWA (75 : 24 : 1, isopropanol : water : acetic acid), MWA (75 : 24 : 1, methanol : water : acetic acid), PA (99 : 1, isopropanol : acetic acid), and MA (99 : 1, methanol : acetic acid)] from control and drought-stressed A. thaliana rosette leaves. “FW” represents fresh weight of plant sample. Asterisks denote a significant hormonal difference between control and drought-stressed plants and “nd” refers to not detected (n = 2/3, t-test, P < 0.1, ∗∗ P < 0.05).
Figure 5
Figure 5
Hormonal levels from normal and salt-stressed A. thaliana rosette leaves samples after extraction using solvent methanol : acetic acid (99 : 1, v/v). “FW” represents fresh weight of plant sample. Asterisks indicate significant differences and “nd” refers to not detected (n = 2/3, P < 0.05, Student's t-test).

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References

    1. Peleg Z., Blumwald E. Hormone balance and abiotic stress tolerance in crop plants. Current Opinion in Plant Biology. 2011;14(3):290–295. doi: 10.1016/j.pbi.2011.02.001. - DOI - PubMed
    1. Daszkowska-Golec A., Szarejko I. Open or close the gate—stomata action under the control of phytohormones in drought stress conditions. Frontiers in Plant Science. 2013;4(138):1–16. doi: 10.3389/fpls.2013.00138. - DOI - PMC - PubMed
    1. Miura K., Tada Y. Regulation of water, salinity, and cold stress responses by salicylic acid. Frontiers in Plant Science. 2014;5(4):12. doi: 10.3389/fpls.2014.00004. - DOI - PMC - PubMed
    1. Fariduddin Q., Yusuf M., Ahmad I., Ahmad A. Brassinosteroids and their role in response of plants to abiotic stresses. Biologia Plantarum. 2013;58(1):9–17. doi: 10.1007/s10535-013-0374-5. - DOI
    1. Divi U. K., Rahman T., Krishna P. Berseaarschs airnticolesteroid-mediated stress tolerance in Arabidopsis shows interactions with abscisic acid, ethylene and salicylic acid pathways. BMC Plant Biology. 2010;10, article 151 doi: 10.1186/1471-2229-10-151. - DOI - PMC - PubMed

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