A randomized controlled experimental study of the efficacy of platelet-rich plasma and hyaluronic acid for the prevention of adhesion formation in a rat uterine horn model

Arch Gynecol Obstet. 2016 Sep;294(3):533-40. doi: 10.1007/s00404-016-4079-9. Epub 2016 Mar 29.


Purpose: Platelet-rich plasma (PRP) has been known to possess an efficacy in tissue regeneration. The aim of this study was to determine the role of PRP on post-operative adhesion formation in an experimental rat study.

Methods: Thirty Sprague-Dawley rats were randomly divided into control, hyaluronic acid, and PRP treatment groups and operated on for uterine horn adhesion modeling. Blood was collected to produce a PRP with platelet counts of 688 × 10(3)/μL, and 1 ml of either hyaluronic acid gel or PRP was administered over the standard lesions, while the control group received no medication. The evaluation of post-operative adhesions was done on the 30th post-operative day. The location, extent, type, and tenacity of adhesions as well as total adhesion scores, tissue inflammation, fibrosis and transforming growth factor-1beta (TGF-1β) expressions were evaluated.

Results: The total adhesion score was significantly lower in the PRP group (3.2 ± 1.5) compared with the hyaluronic acid (5.0 ± 1.3) and control (8.1 ± 1.7) groups. The extent of the adhesions was significantly lower in the PRP group. There was no significant difference in the type and tenacity of adhesions between the hyaluronic acid and the PRP group. The level of inflammation was significantly higher in the control group than the others, while there was no difference between the PRP and hyaluronic acid groups. TGF-1β expression was significantly lesser in the PRP group than the control and hyaluronic acid groups.

Conclusions: PRP is more effective than hyaluronic acid treatment in preventing post-operative adhesion formation in an experimental rat uterine horn adhesion model.

Keywords: Experimental rat model; Platelet-rich plasma; Transforming growth factor-1β; Uterine horn adhesion model.

MeSH terms

  • Animals
  • Disease Models, Animal
  • Female
  • Hyaluronic Acid / therapeutic use*
  • Platelet Count
  • Platelet-Rich Plasma*
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Tissue Adhesions / prevention & control*
  • Uterine Diseases / prevention & control*


  • Hyaluronic Acid