Endothelin-1 Induces Proteinuria by Heparanase-Mediated Disruption of the Glomerular Glycocalyx

J Am Soc Nephrol. 2016 Dec;27(12):3545-3551. doi: 10.1681/ASN.2015091070. Epub 2016 Mar 29.

Abstract

Diabetic nephropathy (DN) is the leading cause of CKD in the Western world. Endothelin receptor antagonists have emerged as a novel treatment for DN, but the mechanisms underlying the protective effect remain unknown. We previously showed that both heparanase and endothelin-1 are essential for the development of DN. Here, we further investigated the role of these proteins in DN, and demonstrated that endothelin-1 activates podocytes to release heparanase. Furthermore, conditioned podocyte culture medium increased glomerular transendothelial albumin passage in a heparanase-dependent manner. In mice, podocyte-specific knockout of the endothelin receptor prevented the diabetes-induced increase in glomerular heparanase expression, consequent reduction in heparan sulfate expression and endothelial glycocalyx thickness, and development of proteinuria observed in wild-type counterparts. Our data suggest that in diabetes, endothelin-1 signaling, as occurs in endothelial activation, induces heparanase expression in the podocyte, damage to the glycocalyx, proteinuria, and renal failure. Thus, prevention of these effects may constitute the mechanism of action of endothelin receptor blockers in DN.

Keywords: diabetic nephropathy; glomerular endothelial cells; glomerular filtration barrier; podocyte; proteinuria.

MeSH terms

  • Animals
  • Diabetic Nephropathies / etiology
  • Endothelin-1 / physiology*
  • Glucuronidase / physiology*
  • Glycocalyx / enzymology*
  • Kidney Glomerulus / enzymology*
  • Kidney Glomerulus / ultrastructure*
  • Male
  • Mice
  • Podocytes / enzymology
  • Proteinuria / etiology*

Substances

  • Endothelin-1
  • heparanase
  • Glucuronidase