Integrated analysis of miRNAs and transcriptomes in Aedes albopictus midgut reveals the differential expression profiles of immune-related genes during dengue virus serotype-2 infection

Insect Sci. 2016 Jun;23(3):377-85. doi: 10.1111/1744-7917.12339. Epub 2016 May 16.


Mosquito microRNAs (miRNAs) are involved in host-virus interaction, and have been reported to be altered by dengue virus (DENV) infection in Aedes albopictus (Diptera: Culicidae). However, little is known about the molecular mechanisms of Aedes albopictus midgut-the first organ to interact with DENV-involved in its resistance to DENV. Here we used high-throughput sequencing to characterize miRNA and messenger RNA (mRNA) expression patterns in Aedes albopictus midgut in response to dengue virus serotype 2. A total of three miRNAs and 777 mRNAs were identified to be differentially expressed upon DENV infection. For the mRNAs, we identified 198 immune-related genes and 31 of them were differentially expressed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses also showed that the differentially expressed immune-related genes were involved in immune response. Then the differential expression patterns of six immune-related genes and three miRNAs were confirmed by real-time reverse transcription polymerase chain reaction. Furthermore, seven known miRNA-mRNA interaction pairs were identified by aligning our two datasets. These analyses of miRNA and mRNA transcriptomes provide valuable information for uncovering the DENV response genes and provide a basis for future study of the resistance mechanisms in Aedes albopictus midgut.

Keywords: Aedes albopictus; DENV; host-pathogen interaction; mRNA; miRNA; midgut.

MeSH terms

  • Aedes / genetics
  • Aedes / immunology
  • Aedes / virology*
  • Animals
  • Dengue Virus / physiology*
  • Gastrointestinal Tract / immunology
  • Gastrointestinal Tract / metabolism
  • MicroRNAs / genetics*
  • RNA, Messenger / genetics
  • Transcriptome*


  • MicroRNAs
  • RNA, Messenger