Effective microorganism - X attenuates circulating superoxide dismutase following an acute bout of intermittent running in hot, humid conditions

Res Sports Med. 2016 Apr-Jun;24(2):130-44. doi: 10.1080/15438627.2015.1126279. Epub 2016 Mar 31.

Abstract

This study determined the effectiveness of antioxidant supplementation on high-intensity exercise-heat stress. Six males completed a high-intensity running protocol twice in temperate conditions (TEMP; 20.4°C), and twice in hot conditions (HOT; 34.7°C). Trials were completed following7 days supplementation with 70 ml·day(-1) effective microorganism-X (EM-X; TEMPEMX or HOTEMX) or placebo (TEMPPLA or HOTPLA). Plasma extracellular Hsp72 (eHsp72) and superoxide dismutase (SOD) were measured by ELISA. eHsp72 and SOD increased pre-post exercise (p < 0.001), with greater eHsp72 (p < 0.001) increases observed in HOT (+1.5 ng·ml(-1)) compared to TEMP (+0.8 ng·ml(-1)). EM-X did not influence eHsp72 (p > 0.05). Greater (p < 0.001) SOD increases were observed in HOT (+0.22 U·ml(-1)) versus TEMP (+0.10 U·ml(-1)) with SOD reduced in HOTEMX versus HOTPLA (p = 0.001). Physiological and perceptual responses were all greater (p < 0.001) in HOT versus TEMP conditions, with no difference followed EM-X (p > 0.05). EM-X supplementation attenuated the SOD increases following HOT, potentiating its application as an ergogenic aid to ameliorate oxidative stress.

Keywords: HIT; exercise; heat shock proteins (HSP); human; oxidative stress; redox balance; repeated sprint.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Antioxidants / administration & dosage
  • Antioxidants / pharmacology*
  • Biomarkers / blood
  • Cross-Over Studies
  • Dietary Supplements
  • Double-Blind Method
  • HSP72 Heat-Shock Proteins / blood
  • Heat-Shock Response / drug effects*
  • Heat-Shock Response / physiology
  • Hot Temperature / adverse effects*
  • Humans
  • Humidity / adverse effects*
  • Linear Models
  • Male
  • Oxidative Stress / drug effects*
  • Oxidative Stress / physiology
  • Running / physiology*
  • Superoxide Dismutase / blood*
  • Young Adult

Substances

  • Antioxidants
  • Biomarkers
  • HSP72 Heat-Shock Proteins
  • Superoxide Dismutase