Affinity purification lipase from wheat germ: comparison of hydrophobic and metal chelation effect

Artif Cells Nanomed Biotechnol. 2017 May;45(3):574-583. doi: 10.3109/21691401.2016.1161642. Epub 2016 Mar 31.

Abstract

Cryogels are used quite a lot nowadays for adsorption studies as synthetic adsorbents. In this study, lipase enzyme (obtained from Candida cylindracea) adsorption capacity of poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan), poly(HEMA-MATrp), and Cu(II) ions immobilized poly(HEMA-MATrp), poly(HEMA-MATrp)-Cu(II), cryogel membranes were synthesized to determine and compare the adsorption behavior of lipase enzyme. In this regard, the effect of pH, interaction time, lipase initial concentration, temperature and ionic strength on the adsorption capacity of these membranes was investigated. Maximum lipase enzyme adsorption capacities of poly(HEMA-MATrp) and poly(HEMA-MATrp)-Cu(II) cryogel membranes were determined as 166.4 mg/g and 196.4 mg/g, respectively.

Keywords: Adsorption; cryogel; enzyme; lipase; membrane.

Publication types

  • Comparative Study

MeSH terms

  • Adsorption
  • Candida / chemistry*
  • Candida / enzymology
  • Cations, Divalent
  • Chelating Agents / chemistry
  • Chromatography, Affinity / methods
  • Copper / chemistry
  • Cryogels / chemistry*
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / isolation & purification*
  • Fungal Proteins / chemistry
  • Fungal Proteins / isolation & purification*
  • Hydrogen-Ion Concentration
  • Hydrophobic and Hydrophilic Interactions
  • Lipase / chemistry
  • Lipase / isolation & purification*
  • Osmolar Concentration
  • Polymethacrylic Acids / chemistry*
  • Temperature
  • Triticum / microbiology
  • Tryptophan / analogs & derivatives*
  • Tryptophan / chemistry

Substances

  • Cations, Divalent
  • Chelating Agents
  • Cryogels
  • Enzymes, Immobilized
  • Fungal Proteins
  • Polymethacrylic Acids
  • poly(hydroxyethyl methacrylate-N-methacryloyltyroptophan methylester)
  • Copper
  • Tryptophan
  • Lipase