Myosin II governs collective cell migration behaviour downstream of guidance receptor signalling

J Cell Sci. 2017 Jan 1;130(1):97-103. doi: 10.1242/jcs.179952. Epub 2016 Mar 31.


Border cell migration during Drosophila oogenesis is a potent model to study collective cell migration, a process involved in development and metastasis. Border cell clusters adopt two main types of behaviour during migration: linear and rotational. However, the molecular mechanism controlling the switch from one to the other is unknown. Here, we demonstrate that non-muscle Myosin II (NMII, also known as Spaghetti squash) activity controls the linear-to-rotational switch. Furthermore, we show that the regulation of NMII takes place downstream of guidance receptor signalling and is critical to ensure efficient collective migration. This study thus provides new insight into the molecular mechanism coordinating the different cell behaviours in a migrating cluster.

Keywords: Border cells; Collective cell migration; Guidance receptor; Myosin II.

MeSH terms

  • Animals
  • Cell Movement*
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / cytology*
  • Drosophila melanogaster / metabolism*
  • ErbB Receptors / metabolism
  • Myosin Type II / metabolism*
  • Receptor Protein-Tyrosine Kinases / metabolism*
  • Rotation
  • Signal Transduction*
  • Time-Lapse Imaging


  • Drosophila Proteins
  • Sqh protein, Drosophila
  • ErbB Receptors
  • Pvr protein, Drosophila
  • Receptor Protein-Tyrosine Kinases
  • Myosin Type II