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. 2016 Apr 1;84:A.3A.1-A.3A.8.
doi: 10.1002/0471140864.psa03as84.

Use of Protein Folding Reagents

No authors listed

Use of Protein Folding Reagents

No authors listed. Curr Protoc Protein Sci. .


The reagents and methods for purification and use of the most commonly used denaturants, guanidine hydrochloride (guanidine-HCl) and urea, are described. Other protein denaturants and reagents used to fold proteins are briefly mentioned. Sulfhydryl reagents (reducing agents) and "oxido-shuffling" (or oxidative regeneration) systems are also described.

Keywords: guanidine hydrochloride; oxidative regeneration; protein denaturants; protein folding; sulfhydryl reagents; urea.

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