Collagencin, an antibacterial peptide from fish collagen: Activity, structure and interaction dynamics with membrane

Biochem Biophys Res Commun. 2016 Apr 29;473(2):642-7. doi: 10.1016/j.bbrc.2016.03.121. Epub 2016 Mar 30.


In this study, we first report characterization of collagencin, an antimicrobial peptide identified from fish collagen hydrolysate. The peptide completely inhibited the growth of Staphylococcus aureus at 1.88 mM. Although non-toxic up to 470 μM, collagencin was hemolytic at higher concentrations. The secondary structure of collagencin was mainly composed by β-sheet and β-turn as determined by CD measurements and molecular dynamics. The peptide is likely to form β-sheet structure under hydrophobic environments and interacts with both anionic (phosphatidylglycerol) and zwitterionic (phosphoethanolamine and phosphatidylcholine) lipids as shown with CD spectroscopy and molecular dynamics. The peptide formed several hydrogen bonds with both POPG and POPE lipids and remained at membrane-water interface, suggesting that collagencin antibacterial action follows a carpet mechanism. Collagenous fish wastes could be processed by enzymatic hydrolysis and transformed into products of high value having functional or biological properties. Marine collagens are a promising source of antimicrobial peptides with new implications in food safety and human health.

Keywords: Antibacterial activity; Circular dichroism; Collagencin; Fish collagen; Mechanism of action; Molecular dynamics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / pharmacology*
  • Antimicrobial Cationic Peptides / chemistry*
  • Antimicrobial Cationic Peptides / pharmacology*
  • Circular Dichroism
  • Collagen / chemistry*
  • Collagen / pharmacology*
  • Fishes / metabolism
  • Humans
  • Membrane Lipids / metabolism
  • Molecular Dynamics Simulation
  • Protein Structure, Secondary
  • Staphylococcal Infections / drug therapy
  • Staphylococcus aureus / drug effects


  • Anti-Bacterial Agents
  • Antimicrobial Cationic Peptides
  • Membrane Lipids
  • Collagen