miR-601 is a prognostic marker and suppresses cell growth and invasion by targeting PTP4A1 in breast cancer

Biomed Pharmacother. 2016 Apr;79:247-53. doi: 10.1016/j.biopha.2016.02.014. Epub 2016 Mar 11.

Abstract

MicroRNAs (miRNA) play important roles in the initiation and progression of breast cancer. Here, we investigated the role of miR-601 in breast cancer and found that its expression was significantly down-regulated in breast cancer tissues compared with matched adjacent non-cancerous breast tissues. Moreover, we found that down-regulation of miR-601 was closely associated with distant metastasis and poor distant metastasis-free survival in breast cancer. In addition, miR-601 levels were inversely correlated with metastatic potential of human breast cancer cell lines. Further experiments showed that ectopic overexpression of miR-601 suppressed breast cancer cell proliferation, migration and invasion, whereas miR-601 knockdown promoted breast cancer cell proliferation, migration and invasion. Furthermore, protein tyrosine phosphatase type IVA 1 (PTP4A1) was identified as a direct target of miR-601. Overexpression of miR-601 repressed PTP4A1 mRNA and protein expression. Conversely, inhibition of miR-601 increased PTP4A1 mRNA and protein expression. Taken together, our data suggest that miR-601 inhibits growth and invasion of breast cancer cells by targeting PTP4A1 and that miR-601 is a potential biomarker for prognosis and therapeutic target in breast cancer.

Keywords: Breast cancer; Metastasis; PTP4A1; Prognosis; miR-601.

MeSH terms

  • Base Sequence
  • Biomarkers, Tumor / metabolism*
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology*
  • Cell Cycle Proteins / metabolism*
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Disease-Free Survival
  • Down-Regulation
  • Female
  • Humans
  • Membrane Proteins / metabolism*
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Neoplasm Invasiveness
  • Prognosis
  • Protein Tyrosine Phosphatases / metabolism*
  • Tumor Stem Cell Assay

Substances

  • Biomarkers, Tumor
  • Cell Cycle Proteins
  • MIRN601 microRNA, human
  • Membrane Proteins
  • MicroRNAs
  • PTP4A1 protein, human
  • Protein Tyrosine Phosphatases