Ideonella sakaiensis sp. nov., isolated from a microbial consortium that degrades poly(ethylene terephthalate)

Int J Syst Evol Microbiol. 2016 Aug;66(8):2813-2818. doi: 10.1099/ijsem.0.001058. Epub 2016 Apr 5.


A Gram-stain-negative, aerobic, non-spore-forming, rod-shaped bacterium, designed strain 201-F6T, was isolated from a microbial consortium that degrades poly(ethylene terephthalate) (PET) collected in Sakai city, Japan, and was characterized on the basis of a polyphasic taxonomic study. The cells were motile with a polar flagellum. The strain contained cytochrome oxidase and catalase. It grew within the pH range 5.5-9.0 (optimally at pH 7-7.5) and at 15-42 ºC (optimally at 30-37 ºC). The major isoprenoid quinone was ubiquinone with eight isoprene units (Q-8). C16 : 0, C17 : 0 cyclo, C18 :1ω7c and C12 : 0 2-OH were the predominant cellular fatty acids. The major polar lipids were phosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of genomic DNA was 70.4 mol%. Phylogenetic analysis using the 16S rRNA gene sequences showed that strain 201-F6T was affiliated to the genus Ideonella, and was closely related to Ideonella dechloratans LMG 28178T (97.7 %) and Ideonella azotifigens JCM 15503T (96.6 %). Strain 201-F6T could be clearly distinguished from the related species of the genus Ideonella by its physiological and biochemical characteristics as well as by its phylogenetic position and DNA-DNA relatedness. Therefore, the strain represents a novel species of the genus Ideonella, for which the name Ideonella sakaiensis sp. nov. (type strain 201-F6T=NBRC 110686T=TISTR 2288T) is proposed.

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Betaproteobacteria / classification*
  • Betaproteobacteria / genetics
  • Betaproteobacteria / isolation & purification
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Japan
  • Microbial Consortia*
  • Nucleic Acid Hybridization
  • Phospholipids / chemistry
  • Phylogeny*
  • Polyethylene Terephthalates / chemistry*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Ubiquinone / chemistry


  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • Polyethylene Terephthalates
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • ubiquinone 8