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, 39, 114-6

A T-RFLP Database for the Rapid Profiling of Methanogenic Communities in Anaerobic Digesters

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A T-RFLP Database for the Rapid Profiling of Methanogenic Communities in Anaerobic Digesters

Franziska Bühligen et al. Anaerobe.

Abstract

We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing.

Keywords: Biogas; BstNI; Community fingerprinting; Methanogenic archaea; MwoI; mcrA.

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