A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters

Franziska Bühligen; Rico Lucas; Marcell Nikolausz; Sabine Kleinsteuber

doi:10.1016/j.anaerobe.2016.03.013

A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters

Anaerobe. 2016 Jun:39:114-6. doi: 10.1016/j.anaerobe.2016.03.013. Epub 2016 Apr 1.

Authors

Franziska Bühligen¹, Rico Lucas¹, Marcell Nikolausz¹, Sabine Kleinsteuber²

Affiliations

¹ UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany.
² UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany. Electronic address: sabine.kleinsteuber@ufz.de.

PMID: 27046270
DOI: 10.1016/j.anaerobe.2016.03.013

Abstract

We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing.

Keywords: Biogas; BstNI; Community fingerprinting; Methanogenic archaea; MwoI; mcrA.

MeSH terms

Anaerobiosis / genetics*
Archaea / classification*
Archaea / genetics*
Archaea / metabolism
Biofuels / analysis
Bioreactors
DNA Fingerprinting
DNA Restriction Enzymes / genetics
DNA Restriction Enzymes / metabolism
Databases, Genetic*
Deoxyribonucleases, Type II Site-Specific / genetics
Deoxyribonucleases, Type II Site-Specific / metabolism
Methane / biosynthesis*
Phylogeny*
Polymorphism, Restriction Fragment Length

Substances

Biofuels
DNA Restriction Enzymes
endodeoxyribonuclease McrA
endodeoxyribonuclease MwoI
CCWGG-specific type II deoxyribonucleases
Deoxyribonucleases, Type II Site-Specific
Methane