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, 8 (10), 1230-6

Evaluation of Antigout Activity of Phyllanthus Emblica Fruit Extracts on Potassium Oxonate-Induced Gout Rat Model

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Evaluation of Antigout Activity of Phyllanthus Emblica Fruit Extracts on Potassium Oxonate-Induced Gout Rat Model

Vaidehi N Sarvaiya et al. Vet World.

Abstract

Aim: The present study has been conducted to evaluate antigout activity of aqueous and alcoholic extracts of Phyllanthus emblica fruits following its 28 days repeated oral administration on potassium oxonate-induced gout rat model.

Materials and methods: The study was conducted on 42 male Sprague-Dawely rats dividing them in seven groups having six rats in each group. Groups I, II, and III served as vehicle control group, gout control group, and standard treatment control group, respectively. Rats of all the groups except vehicle control group were administered potassium oxonate at 250 mg/kg (IP), throughout the study period (28 days) for induction of gout. Groups IV and V received aqueous extract of P. emblica at 200 and 400 mg/kg, and Groups VI and VII received alcoholic extract of P. emblica at 200 and 400 mg/kg (daily oral for 28 days). At the end of study, all the rats were subjected to blood collection; blood and serum sample were analyzed for hematological and biochemical parameters, respectively. After collection of blood samples on the 29(th) day, all the rats were sacrificed and subjected to post mortem examination to determine the presence or absence of gross and histopathological lesions in kidney tissues.

Results: At the end of study, rats of gout control group showed increase in platelets counts, serum creatinine, uric acid, blood urea nitrogen (BUN), and xanthine oxidase (XO) enzyme level along with alterations in kidney tissues as compared to vehicle control group. Gouty rats treated with aqueous and alcoholic extracts of P. emblica at 200 and 400 mg/kg body weight and standard treatment allopurinol at 5 mg/kg body weight showed reduction in platelets counts, serum creatinine, uric acid, BUN, and XO enzyme level along with significant improvements in histological structure of kidney as compared to rats of gout control group.

Conclusion: Oral administration of aqueous and alcoholic extracts of P. emblica fruits for 28 days has shown protection against gout in dose-dependent manner in rats.

Keywords: Phyllanthus emblica fruits; antigout activity; gout rat model; potassium oxonate.

Figures

Figure-1
Figure-1
Photograph showing fruits of Phyllanthus emblica (Amla).
Figure-2
Figure-2
Section of kidney of rat from gout control group (Group II) showing atrophy of glomeruli, severe congestion and hemorrhage with degeneration and necrosis of renal tubular epithelium (H and E,×240).
Figure-3
Figure-3
Section of kidney of rat from gout control group (Group II) showing presence of proteinaceous cast in renal tubular lumen (H and E,×240).
Figure-4
Figure-4
Section of kidney from gout control rat (Group II) showing deposition of urate crystals in renal parenchyma (H and E,×240).
Figure-5
Figure-5
Section of kidney from rat of Group VII treated with alcoholic extract of Phyllanthus emblica at 400 mg/kg revealing significant improvements in renal histomorphological structures (H and E,×120).

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