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. 2015 May;8(5):656-63.
doi: 10.14202/vetworld.2015.656-663. Epub 2015 May 26.

Pharmacodynamic Interaction of Fenugreek, Insulin and Glimepiride on Sero-Biochemical Parameters in Diabetic Sprague-Dawley Rats

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Free PMC article

Pharmacodynamic Interaction of Fenugreek, Insulin and Glimepiride on Sero-Biochemical Parameters in Diabetic Sprague-Dawley Rats

C Haritha et al. Vet World. .
Free PMC article

Abstract

Aim: This study was undertaken to assess the pharmacodynamic interaction of fenugreek, insulin and glimepiride on sero-biochemical parameters in streptozotocin-induced diabetic rats.

Materials and methods: A total of 56 male Sprague-Dawley rats, randomly divided into seven Groups. Group 1: Non-diabetic control; Group 2: Streptozotocin induced diabetic control; Groups 3, 4 and 5 were treated with insulin, glimepiride and fenugreek seed powder, respectively; Groups 6 and 7: Insulin + fenugreek seed powder treatment and glimepiride + fenugreek seed powder treatment respectively, in diabetic rats. Body weights, blood glucose, lipids total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides (TG) and proteins (total protein, albumin, globulin, A/G ratios) were studied at different time intervals. Rats were sacrified at the end of 8 weeks, pancreas and aorta collected for histopathological study.

Results: The results of Group 2 showed significantly (p<0.05) higher concentration of glucose, TC, TG, LDL, globulin, A/G ratios and significantly (p<0.05) lower concentration of albumin, total protein, HDL and body weights when compared to Group 1 at the end of 4(th) and 8(th) weeks intervals with marked alteration in histopathology of pancreas and aorta. All the treatment Groups 3-7 showed significantly (p<0.05) improvement in the all the parameters and the Groups 6 and 7 showed highest decrease in the concentration blood glucose, TC, TG, LDL and increase in the albumin, total protein and body weights during 6(th) and 8(th) week, respectively.

Conclusion: The treatment with fenugreek, insulin and glimepiride countered the alteration in the sero biochemical parameters in diabetic rats, and their combination was found a positive interaction in improving the sero biochemical status of diabetic rats.

Keywords: cholesterol; diabetes; fenugreek; glimepiride; glucose; insulin; protein.

Figures

Figure-1
Figure-1
Photomicrograph of pancreas showing marked congestion (C) (H and E, ×200; Group 2).
Figure-2
Figure-2
Photomicrograph of pancreas showing mild congestion (C) and depleted acini (D) (H and E, ×200; Group 2).
Figure-3
Figure-3
Photomicrograph of pancreas showing moderate congestion (C) (H and E, ×200; Group 4).
Figure-4
Figure-4
Photomicrograph of pancreas showing mild congestion (C) (H and E, ×200; Group 7).
Figure-5
Figure-5
Photomicrograph of aorta showing marked congestion (C) in the tunica adventitia with heavy infiltration of lymphocytes (L). (H and E, ×200; Group 2).
Figure-6
Figure-6
Photomicrograph of aorta showing marked congestion (C) between tunica layers (H and E, ×200; Group 3).
Figure-7
Figure-7
Photomicrograph of aorta showing mild congestion (C) in the tunica intima (H and E, ×200; Group 6).
Figure-8
Figure-8
Photomicrograph of aorta showing mild infiltration of lymphocytes (L) (H and E, ×200; Group 7).

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