MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques

Oncotarget. 2017 Feb 21;8(8):12686-12694. doi: 10.18632/oncotarget.8475.


Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease in humans with a case fatality rate of over 39%, and poses a considerable threat to public health. A lack of approved vaccine or drugs currently constitutes a roadblock in controlling disease outbreak and spread. In this study, we generated MERS-CoV VLPs using the baculovirus expression system. Electron microscopy and immunoelectron microscopy results demonstrate that MERS-CoV VLPs are structurally similar to the native virus. Rhesus macaques inoculated with MERS-CoV VLPs and Alum adjuvant induced virus-neutralizing antibodies titers up to 1:40 and induced specific IgG antibodies against the receptor binding domain (RBD), with endpoint titers reaching 1:1,280. MERS-CoV VLPs also elicited T-helper 1 cell (Th1)-mediated immunity, as measured by ELISpot. These data demonstrate that MERS-CoV VLPs have excellent immunogenicity in rhesus macaques, and represent a promising vaccine candidate.

Keywords: Middle East respiratory syndrome coronavirus; immune response; nonhuman primates; vaccine; virus-like particles.

MeSH terms

  • Animals
  • Antibodies, Neutralizing / immunology
  • Antibodies, Viral / immunology
  • Blotting, Western
  • Coronavirus Infections / immunology*
  • Disease Models, Animal
  • Fluorescent Antibody Technique, Indirect
  • Immunity, Cellular / immunology*
  • Immunity, Humoral / immunology*
  • Insecta
  • Macaca mulatta
  • Microscopy, Electron, Transmission
  • Middle East Respiratory Syndrome Coronavirus / immunology*
  • Vaccines, Virus-Like Particle / immunology*
  • Viral Vaccines / immunology*


  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Vaccines, Virus-Like Particle
  • Viral Vaccines