Data on hepatic lipolysis, adipose triglyceride lipase, and hormone-sensitive lipase in fasted and non-fasted C57BL/6J female mice

Phillip M Marvyn; Emily B Mardian; Ryan M Bradley; Kristin A Marks; Robin E Duncan

doi:10.1016/j.dib.2016.03.033

Data on hepatic lipolysis, adipose triglyceride lipase, and hormone-sensitive lipase in fasted and non-fasted C57BL/6J female mice

Data Brief. 2016 Mar 11:7:721-5. doi: 10.1016/j.dib.2016.03.033. eCollection 2016 Jun.

Authors

Phillip M Marvyn¹, Emily B Mardian¹, Ryan M Bradley¹, Kristin A Marks¹, Robin E Duncan¹

Affiliation

¹ University of Waterloo, Department of Kinesiology, Faculty of Applied Health Sciences, 200 University Avenue W., BMH1110, Waterloo, Ontario, Canada N2L 3G1.

Abstract

Liver homogenates produced from fasted and non-fasted C57BL/6J female mice were assayed for total lipolytic activity measured as hydrolysis of [9,10-(3)H(N)]-triolein into [(3)H] free fatty acids (FFA). Liver homogenates were also used for immunoblotting to determine levels of the lipolytic enzymes adipose-triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), as well as site specific phosphorylation at the 14-3-3 binding site of ATGL and the serine 565 and serine 660 sites of HSL. Significantly higher triolein hydrolysis activity was observed in fasted liver samples, as well as a significant increase in total ATGL and a significant decrease in HSL phosphorylation at the S565 site.