CKAP2 is necessary to ensure the faithful spindle bipolarity in a dividing diploid hepatocyte

Biochem Biophys Res Commun. 2016 May 13;473(4):886-893. doi: 10.1016/j.bbrc.2016.03.145. Epub 2016 Apr 4.


Spindle bipolarity is crucial for segregating chromosome during somatic cell division. Previous studies have suggested that cytoskeleton associated protein 2 (CKAP2) is involved in spindle assembly and chromosome segregation. In this study, we show that CKAP2-depleted primary hepatocytes exhibit over-duplicated centrosomes with disjoined chromosomes from metaphase plate. These cells proceed to apoptosis or multipolar cell division and subsequent apoptotic cell death. In addition, a mouse liver regeneration experiment showed a marked decrease in efficiency of hepatic regeneration in CKAP2-depleted liver. These data suggest a physiological role of CKAP2 in the formation of spindle bipolarity, which is necessary for maintaining chromosomal stability.

Keywords: CKAP2; Centrosome; Chromosome segregation; Multipolar cell division.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Division / genetics*
  • Cells, Cultured
  • Centrosome / physiology
  • Chromosome Segregation / genetics
  • Cytoskeletal Proteins / metabolism*
  • Diploidy
  • Hepatocytes / cytology*
  • M Phase Cell Cycle Checkpoints / genetics
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Spindle Apparatus / genetics*


  • CKAP2 protein, mouse
  • Cytoskeletal Proteins