Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission

Elife. 2016 Apr 8;5:e15075. doi: 10.7554/eLife.15075.

Abstract

Plants constantly renew during their life cycle and thus require to shed senescent and damaged organs. Floral abscission is controlled by the leucine-rich repeat receptor kinase (LRR-RK) HAESA and the peptide hormone IDA. It is unknown how expression of IDA in the abscission zone leads to HAESA activation. Here we show that IDA is sensed directly by the HAESA ectodomain. Crystal structures of HAESA in complex with IDA reveal a hormone binding pocket that accommodates an active dodecamer peptide. A central hydroxyproline residue anchors IDA to the receptor. The HAESA co-receptor SERK1, a positive regulator of the floral abscission pathway, allows for high-affinity sensing of the peptide hormone by binding to an Arg-His-Asn motif in IDA. This sequence pattern is conserved among diverse plant peptides, suggesting that plant peptide hormone receptors may share a common ligand binding mode and activation mechanism.

Keywords: a. thaliana; biophysics; floral abscission; membrane signaling; peptide hormone; plant biology; plant development; protein complexes; receptor kinase; structural biology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / physiology*
  • Arabidopsis Proteins / biosynthesis*
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / metabolism*
  • Binding Sites
  • Crystallography, X-Ray
  • Flowers / physiology*
  • Gene Expression Regulation, Plant*
  • Protein Binding
  • Protein Conformation
  • Protein Kinases / metabolism*
  • Protein-Serine-Threonine Kinases / biosynthesis*
  • Protein-Serine-Threonine Kinases / chemistry
  • Signal Transduction*

Substances

  • Arabidopsis Proteins
  • IDA protein, Arabidopsis
  • Protein Kinases
  • RLK5 protein, Arabidopsis
  • SERK1 protein, Arabidopsis
  • Protein-Serine-Threonine Kinases

Grant support

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.