Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100

Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29.


O-polysaccharides of E. coli O28ab, O37, and O100 were found to contain glycerol 1-phosphate and the following structures of their oligosaccharide repeats were established by sugar analysis, Smith degradation (for O28ab), 1D and 2D (1)H, (13)C, and (13)P NMR spectroscopy: [Formula: see text]. Functions of putative glycosyltransferases genes in the O-antigen gene clusters of the strains studied were tentatively assigned based on similarities to genes of other E. coli O-serogroups available from GenBank and taking into account the O-polysaccharide structures established.

Keywords: Bacterial polysaccharide structure; Escherichia coli; Glycerol 1-phosphate; Lipopolysaccharide; O-antigen gene cluster; O-polysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Escherichia coli / chemistry*
  • Escherichia coli / classification
  • Escherichia coli / genetics*
  • Glycerophosphates / biosynthesis*
  • Glycerophosphates / chemistry
  • Polysaccharides / biosynthesis*
  • Polysaccharides / chemistry*


  • Glycerophosphates
  • Polysaccharides
  • alpha-glycerophosphoric acid