Transcribing RNA polymerase III observed by electron cryomicroscopy

Niklas A Hoffmann; Arjen J Jakobi; Matthias K Vorländer; Carsten Sachse; Christoph W Müller

doi:10.1111/febs.13732

Transcribing RNA polymerase III observed by electron cryomicroscopy

FEBS J. 2016 Aug;283(15):2811-9. doi: 10.1111/febs.13732. Epub 2016 Apr 28.

Authors

Niklas A Hoffmann¹, Arjen J Jakobi¹, Matthias K Vorländer¹, Carsten Sachse¹, Christoph W Müller¹

Affiliation

¹ European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Heidelberg, Germany.

Abstract

Electron cryomicroscopy reconstructions of elongating RNA polymerase (Pol) III at 3.9 Å resolution and of unbound Pol III (apo Pol III) in two distinct conformations at 4.6 Å and 4.7 Å resolution allow the construction of complete atomic models of Pol III and provide new functional insights into the adaption of Pol III to fulfill its specific transcription tasks.

Keywords: Pol III; RNA polymerase III; electron cryomicroscopy; tRNA; transcription.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Cryoelectron Microscopy
Humans
Models, Molecular
RNA Polymerase III / antagonists & inhibitors
RNA Polymerase III / chemistry*
RNA Polymerase III / metabolism
RNA Polymerase III / ultrastructure
Transcription, Genetic*

Substances

RNA Polymerase III