The regulatory niche of intestinal stem cells

J Physiol. 2016 Sep 1;594(17):4827-36. doi: 10.1113/JP271931. Epub 2016 Jul 28.


The niche constitutes a unique category of cells that support the microenvironment for the maintenance and self-renewal of stem cells. Intestinal stem cells reside at the base of the crypt, which contains adjacent epithelial cells, stromal cells and smooth muscle cells, and soluble and cell-associated growth and differentiation factors. We summarize here recent advances in our understanding of the crucial role of the niche in regulating stem cells. The stem cell niche maintains a balance among quiescence, proliferation and regeneration of intestinal stem cells after injury. Mesenchymal cells, Paneth cells, immune cells, endothelial cells and neural cells are important regulatory components that secrete niche ligands, growth factors and cytokines. Intestinal homeostasis is regulated by niche signalling pathways, specifically Wnt, bone morphogenetic protein, Notch and epidermal growth factor. These insights into the regulatory stem cell niche during homeostasis and post-injury regeneration offer the potential to accelerate development of therapies for intestine-related disorders.

Keywords: Intestinal stem cells; Paneth cells; Signaling pathways; Stem cell niche; Stromal cells; endothelial cell; immune response; neural cells.

Publication types

  • Review
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Endothelial Cells / physiology
  • Intestines / cytology*
  • Neurons / physiology
  • Regeneration
  • Signal Transduction
  • Stem Cell Niche*
  • Stem Cells / physiology*