Reproducibility of Brachial Vascular Changes with Alterations in End-Tidal Carbon Dioxide

Justin R Geijer; Nicholas G Evanoff; Aaron S Kelly; Michael A Chernin; Matthew G Stoltman; Donald R Dengel

doi:10.1016/j.ultrasmedbio.2016.02.003

Reproducibility of Brachial Vascular Changes with Alterations in End-Tidal Carbon Dioxide

Ultrasound Med Biol. 2016 Jul;42(7):1450-6. doi: 10.1016/j.ultrasmedbio.2016.02.003. Epub 2016 Apr 6.

Authors

Justin R Geijer¹, Nicholas G Evanoff², Aaron S Kelly³, Michael A Chernin², Matthew G Stoltman², Donald R Dengel⁴

Affiliations

¹ Department of Health and Exercise Rehabilitation Sciences, Winona State University, Winona, Minnesota, USA.
² School of Kinesiology, University of Minnesota, Minneapolis, Minnesota, USA.
³ Department of Pediatrics, University of Minnesota, and University of Minnesota Masonic Children's Hospital, Minneapolis, Minnesota, USA; Department of Medicine, University of Minnesota, Minneapolis, Minnesota, USA.
⁴ School of Kinesiology, University of Minnesota, Minneapolis, Minnesota, USA; Department of Pediatrics, University of Minnesota, and University of Minnesota Masonic Children's Hospital, Minneapolis, Minnesota, USA. Electronic address: denge001@umn.edu.

PMID: 27061149
DOI: 10.1016/j.ultrasmedbio.2016.02.003

Abstract

The purpose of this study was to examine the reproducibility of the peripheral vascular response to hypercapnia. Healthy college-aged men (n = 7) and women (n = 10) underwent an iso-oxic 10-mm Hg increase in PetCO2 for 12 min. Brachial artery diameter changes were measured using ultrasound imaging. Two tests were completed on day 1 with 15 min of rest between tests. Tests were repeated on day 2. Paired t-tests, Bland-Altman plots and intra-class correlations (ICCs) determined reproducibility. There were no significant differences in peak dilation within day (5.33 ± 3.73% vs. 4.52 ± 2.49%, p = 0.378). The within-day ICC was poor (0.213). Within-day time-to-peak dilation did not significantly differ (660.0 ± 231.8 s vs. 602.7 ± 259.9 s, p = 0.379), and the ICC was fair (0.416, p = 0.113). Between-day peak dilation did not significantly differ (5.24 ± 3.84% vs. 4.71 ± 3.17%, p = 0.123), and the ICC was fair (0.419). Hypercapnia-induced brachial artery dilation is similar within day and between days. The ICC for peak dilation suggests the methodology is not reproducible.

Keywords: Hypercapnia; Ultrasound; Vasodilation.

MeSH terms

Adult
Brachial Artery / diagnostic imaging*
Brachial Artery / physiopathology*
Carbon Dioxide / blood*
Female
Humans
Hypercapnia / physiopathology*
Male
Reproducibility of Results
Ultrasonography / methods*
Young Adult

Substances

Carbon Dioxide