Cycloheximide-sensitive [35S]methionine labeling of proteins in goldfish retinal ganglion cell axons in vitro

Brain Res. 1989 Feb 27;481(1):119-23. doi: 10.1016/0006-8993(89)90491-5.


Polypeptides of retinal ganglion cell axons of the goldfish, regenerating in culture, were labeled by [35S]methionine after decentralization from the explant. Microscopic samples, composed of isolated axonal fields, were analyzed by SDS ultramicroelectrophoresis and autoradiography. Of the several proteins exhibiting cycloheximide-sensitive labeling, beta-tubulin and actin were consistently and prominently labeled, although the possibility of a labeled alpha-isoform with a lower mol. wt. could not be ruled out.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / metabolism*
  • Animals
  • Cells, Cultured
  • Cycloheximide / pharmacology*
  • Cyprinidae / metabolism*
  • Goldfish / metabolism*
  • Methionine / metabolism
  • Nerve Regeneration
  • Nerve Tissue Proteins / metabolism*
  • Retina / metabolism*
  • Retinal Ganglion Cells / cytology
  • Retinal Ganglion Cells / drug effects
  • Retinal Ganglion Cells / metabolism*
  • Tubulin / metabolism*


  • Actins
  • Nerve Tissue Proteins
  • Tubulin
  • Cycloheximide
  • Methionine