Expansion Microscopy With Conventional Antibodies and Fluorescent Proteins

Nat Methods. 2016 Jun;13(6):485-8. doi: 10.1038/nmeth.3833. Epub 2016 Apr 11.

Abstract

Expansion microscopy is a technique in which fluorophores on fixed specimens are linked to a swellable polymer that is physically expanded to enable super-resolution microscopy with ordinary microscopes. We have developed and characterized new methods for linking fluorophores to the polymer that now enable expansion microscopy with conventional fluorescently labeled antibodies and fluorescent proteins. Our methods simplify the procedure and expand the palette of compatible labels, allowing rapid dissemination of the technique.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Brain / ultrastructure
  • Cell Line
  • Image Enhancement / methods*
  • Luminescent Proteins* / genetics
  • Mice, Inbred C57BL
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Staining and Labeling
  • Transfection

Substances

  • Antibodies, Monoclonal
  • Luminescent Proteins