TRPV3 expression and vasodilator function in isolated uterine radial arteries from non-pregnant and pregnant rats

Vascul Pharmacol. 2016 Aug;83:66-77. doi: 10.1016/j.vph.2016.04.004. Epub 2016 Apr 9.

Abstract

This study investigated the expression and function of transient receptor potential vanilloid type-3 ion channels (TRPV3) in uterine radial arteries isolated from non-pregnant and twenty-day pregnant rats. Immunohistochemistry (IHC) suggested TRPV3 is primarily localized to the smooth muscle in arteries from both non-pregnant and pregnant rats. IHC using C' targeted antibody, and qPCR of TRPV3 mRNA, suggested pregnancy increased arterial TRPV3 expression. The TRPV3 activator carvacrol caused endothelium-independent dilation of phenylephrine-constricted radial arteries, with no difference between vessels from non-pregnant and pregnant animals. Carvacrol-induced dilation was reduced by the TRPV3-blockers isopentenyl pyrophosphate and ruthenium red, but not by the TRPA1 or TRPV4 inhibitors HC-030031 or HC-067047, respectively. In radial arteries from non-pregnant rats only, inhibition of NOS and sGC, or PKG, enhanced carvacrol-mediated vasodilation. Carvacrol-induced dilation of arteries from both non-pregnant and pregnant rats was prevented by the IKCa blocker TRAM-34. TRPV3 caused an endothelium-independent, IKCa-mediated dilation of the uterine radial artery. NO-PKG-mediated modulation of TRPV3 activity is lost in pregnancy, but this did not alter the response to carvacrol.

Keywords: Carvacrol; Pregnancy; Radial artery; TRPV3; Uterus.

MeSH terms

  • Animals
  • Blood Pressure
  • Cyclic GMP-Dependent Protein Kinases / metabolism
  • Cymenes
  • Dose-Response Relationship, Drug
  • Female
  • In Vitro Techniques
  • Intermediate-Conductance Calcium-Activated Potassium Channels / metabolism
  • Monoterpenes / pharmacology
  • Nitric Oxide / metabolism
  • Pregnancy
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats, Sprague-Dawley
  • Signal Transduction
  • TRPV Cation Channels / antagonists & inhibitors
  • TRPV Cation Channels / genetics
  • TRPV Cation Channels / metabolism*
  • Up-Regulation
  • Uterine Artery / drug effects
  • Uterine Artery / metabolism*
  • Vasodilation* / drug effects
  • Vasodilator Agents / pharmacology

Substances

  • Cymenes
  • Intermediate-Conductance Calcium-Activated Potassium Channels
  • Monoterpenes
  • RNA, Messenger
  • TRPV Cation Channels
  • TRPV3 protein, rat
  • Vasodilator Agents
  • Nitric Oxide
  • carvacrol
  • Cyclic GMP-Dependent Protein Kinases